NGS Automation Support Program

Optimizing NGS sample preparation steps for automation

Roche Sequencing Solutions allows you tounlock the potential of every sample通过提供自动化脚本,并支持我们的样本准备试剂和工作流程,横跨广泛提供的第三方NGS液体处理平台。

Workflows supporting KAPA DNA and RNA Library Preparation Kits, KAPA Library Quantification Kits and SeqCap target enrichment products are validated and optimized for automated, high-throughput NGS library construction. Workflows for KAPA Target Enrichment are coming soon.

Scripts are currently available for many NGS liquid handling platforms and are continuously being developed to accommodate other Roche Sample Prep Reagents, workflows and liquid handling platforms. In addition, the necessary overage volumes in our 96-reaction kits enable automation-friendly workflows to support your research.

NGS Automation Features and Benefits

Features

  • Validated and supported automation scriptsand workflows from Roche and 3rd-party vendors
  • Streamlined and optimized workflowsfor high performance KAPA DNA or RNA Library Preparation Kits and the HyperCap Workflow using SeqCap target enrichment probes
  • Necessary overage volumes在96-反应试剂盒中,以实现自动化的工作流程

Benefits

  • Higher sample throughput
  • Less hand-on time for greater convenience of batch processing up to 96 samples
  • Greater reproducibility than manual
  • Improved process control
  • Comparable performance with manual methods

自动化方法可用性

The following tables showcase the vendors with which we have partnered to create automation methods. Search the table below by vendor or by product to find information regarding your specific needs. Please contact your local Roche representative for more information regarding our automation methods and for information on products not listed below. Current target enrichment automation methods are for the SeqCap products; KAPA Target Enrichment product automation methods coming soon.

Availability may differ by region. Please contact your local Roche Sales Representative to learn more.

By VendorBy Product

PerkinElmer

Method Source: PerkinElmer

  • KAPA HyperPrep Kit
  • Kapa Hyperplus套件
  • KAPA图书馆量化Kit
  • KAPA Human Genomic DNA Quantification and QC Kit
  • HyperCap Workflow
  • SeqCap EZ Probes
  • KAPA RNA HyperPrep Kit
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)
  • KAPA mRNA HyperPrep Kit

Method Source: PerkinElmer

  • KAPA HyperPrep Kit
  • Kapa Hyperplus套件
  • KAPA图书馆量化Kit
  • KAPA Human Genomic DNA Quantification and QC Kit
  • KAPA RNA HyperPrep Kit
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)
  • KAPA mRNA HyperPrep Kit
  • HyperCap Workflow*


*in development

Method Source: PerkinElmer

  • KAPA HyperPrep Kit
  • Kapa Hyperplus套件
  • KAPA图书馆量化Kit
  • KAPA Human Genomic DNA Quantification and QC Kit

Beckman Coulter

Method Source: Roche

  • KAPA HyperPrep Kit
  • Kapa Hyperplus套件*
  • KAPA图书馆量化Kit
  • KAPA人类基因组DNA定量和QC套件*
  • HyperCap Workflow
  • KAPA RNA HyperPrep Kit*
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)*
  • KAPA mRNA HyperPrep Kit*


*in development

Method Source: Beckman Coulter

  • KAPA图书馆量化Kit

Method Source: Roche

  • Kapa Library量化套件*


*in development

Method Source: Beckman Coulter

  • KAPA HyperPrep Kit*
  • Kapa Hyperplus套件*

*in development

Method Source: Roche

  • KAPA HyperPrep Kit
  • Kapa Hyperplus套件
  • HyperCap Workflow*

*in development

Agilent

Method Source: Agilent

  • KAPA HyperPrep Kit
  • Kapa Hyperplus套件
  • SeqCap EZ Probes


Method Source: Roche

  • Kapa Library量化套件*


*in development

Method Source: Roche

  • KAPA HyperPrep Kit*
  • Kapa Hyperplus套件*
  • KAPA图书馆量化Kit
  • KAPA Human Genomic DNA Quantification and QC Kit
  • Library Normalization*
  • HyperCap Workflow


Method Source: Agilent

  • SeqCap EZ Probes
  • KAPA mRNA HyperPrep Kit

Eppendorf

方法来源:EPPENDORF

  • KAPA HyperPrep Kit
  • Kapa Hyperplus套件
  • KAPA图书馆量化Kit
  • KAPA RNA HyperPrep Kit*
  • KAPA mRNA HyperPrep Kit*


*in development

汉密尔顿

Method Source: Hamilton

  • KAPA HyperPrep Kit
  • Kapa Hyperplus套件
  • KAPA图书馆量化Kit
  • HyperCap Workflow*
  • KAPA RNA HyperPrep Kit*
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)*
  • KAPA mRNA HyperPrep Kit*


*in development

Tecan

Method Source: Tecan

  • KAPA HyperPrep Kit
  • Kapa Hyperplus套件
  • Kapa Library量化套件*
  • SeqCap EZ Probes
  • KAPA RNA HyperPrep Kit*
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)*
  • KAPA mRNA HyperPrep Kit*


*in development

Primadiag.

方法来源:primadiag

  • Kapa Hyperplus套件
  • SeqCap EZ Probes

Brand

方法来源:品牌

  • Kapa Library量化套件*

*in development

By VendorBy Product

KAPA HyperPrep Kit

Method Source: PerkinElmer

  • SciClone G3 NGSX.
  • Zephyr G3 NGS
  • JANUS G3R NGS Express

Method Source: Roche

  • 生物群照片FX.pand FX, dual pod
  • Biomek i7自动工作站

Method Source: Beckman Coulter

  • 生物群照片I5自动工作站*

*in development

Method Source: Agilent

  • BRAVO NGS工作站选项a


Method Source: Roche

  • BRAVO NGS工作站选项B.

方法来源:EPPENDORF

  • epMotion 5075 TMX

Method Source: Hamilton

  • NGS STAR (8 channel)

Method Source: Tecan

  • Freedom Evo NGS Workstation

Kapa Hyperplus套件

Method Source: PerkinElmer

  • SciClone G3 NGSX.
  • Zephyr G3 NGS
  • JANUS G3R NGS Express

Method Source: Roche

  • 生物群照片FX.p and FX, dual pod
  • Biomek i7自动工作站

Method Source: Beckman Coulter

  • 生物群照片I5自动工作站*

*in development

Method Source: Agilent

  • BRAVO NGS工作站选项a


Method Source: Roche

  • BRAVO NGS工作站选项B.



方法来源:EPPENDORF

  • epMotion 5075 TMX

Method Source: Hamilton

  • NGS STAR (8 channel)

Method Source: Tecan

  • Freedom Evo NGS Workstation

方法来源:primadiag

  • Acsia ngs(捕获)

KAPA图书馆量化Kit

Method Source: PerkinElmer

  • SciClone G3 NGSX.
  • Zephyr G3 NGS
  • JANUS G3R NGS Express

Method Source: Roche

  • 生物群照片FX.pand FX, dual pod
  • 生物群照片4000 *


Method Source: Beckman Coulter

  • Biomek NXp
  • Biomek NXp(Span 8)


*in development

Method Source: Roche

  • BRAVO NGS工作站选项a*
  • BRAVO NGS工作站选项B.


*in development

方法来源:EPPENDORF

  • epMotion 5075 TMX

Method Source: Hamilton

  • NGS STAR (8 channel)

Method Source: Tecan

  • Freedom Evo NGS工作站*


*in development

方法来源:品牌

  • 液体搬运站*


*in development

KAPA Human Genomic DNA Quantification and QC Kit

Method Source: PerkinElmer

  • SciClone G3 NGSX.
  • Zephyr G3 NGS
  • JANUS G3R NGS Express



Method Source: Roche

  • 生物群照片FX.p和fx,双pod *


*in development

Method Source: Roche

  • BRAVO NGS工作站选项B.*


*in development

Library Normalization

Method Source: Roche

  • BRAVO NGS工作站选项B.*


*in development

HyperCap Workflow

Method Source: PerkinElmer

  • SciClone G3 NGSX.

Method Source: Roche

  • 生物群照片FX.pand FX, dual pod
  • 生物群照片I7自动工作站*

*in development

Method Source: Roche

  • BRAVO NGS工作站选项B.

Method Source: Hamilton

  • NGS STAR (8 channel)*

*in development

*in development
*in development

SEQCAP EZ工作流程

Method Source: PerkinElmer

  • SciClone G3 NGSX.

Method Source: Agilent

  • BRAVO NGS工作站选项a
  • BRAVO NGS工作站选项B.

Method Source: Tecan

  • Freedom Evo NGS Workstation

方法来源:primadiag

  • Acsia ngs(捕获)

KAPA RNA HyperPrep Kit

Method Source: PerkinElmer

  • SciClone G3 NGSX.
  • Zephyr G3 NGS

Method Source: Roche

  • 生物群照片FX.p和fx,双pod *


*in development

方法来源:EPPENDORF

  • epMotion 5075 TMX*


*in development

Method Source: Hamilton

  • NGS STAR (8 channel)*


*in development

Method Source: Tecan

  • Freedom Evo NGS工作站*


*in development

KAPA RNA HyperPrep Kit with RiboErase (HMR)

Method Source: PerkinElmer

  • SciClone G3 NGSX.
  • Zephyr G3 NGS
  • JANUS G3 R NGS Express

Method Source: Roche

  • 生物群照片FX.p和fx,双pod *


*in development

Method Source: Hamilton

  • NGS STAR (8 channel)*


*in development

Method Source: Tecan

  • Freedom Evo NGS工作站*


*in development

KAPA mRNA HyperPrep Kit

Method Source: PerkinElmer

  • SciClone G3 NGSX.
  • Zephyr G3 NGS
  • JANUS G3 R NGS Express

Method Source: Roche

  • 生物群照片FX.p和fx,双pod *


*in development

Method Source: Agilent

  • BRAVO NGS工作站选项B.

方法来源:EPPENDORF

  • epMotion 5075 TMX*


*in development

Method Source: Hamilton

  • NGS STAR (8 channel)*


*in development

Method Source: Tecan

  • Freedom Evo NGS工作站*


*in development