Overview

KAPA HyperPlus工具包,我们最先进的库准备选项,提供了一个简化的工作流程,包括完全自动化的碎片和单管库准备。这个工具包是基于我们的DNA超组合,提供业界领先的图书馆建设效率。这种综合解决方案结合酶破碎,类似于机械剪切的质量,与基于标签的工作流程的速度和方便。

KAPA HyperPlus Kits offer a complete library preparation solution with KAPA Adapters and KAPA HyperPure Beads (sold separately). The kits are compatible with the Illumina sequencing platform and provide qualified automation methods.

Features and Benefits of KAPA HyperPlus Kits

  • Fully automatable DNA library construction in 1.5-3 hours with low-bias enzymatic fragmentation
  • 业界领先的转化率和文库复杂性,*尤其是FFPE DNA
  • Now includes an additional End Repair and A-Tailing module for more sensitive applications with no change to the workflow
  • 从较低的输入和改进的序列覆盖率,无PCR工作流与PCR工作流
  • 与多种样本类型和输入兼容,在片段大小、适配器设计和库放大方面具有灵活性

Product highlights

具有集成工作流的业界领先的图书馆建设

  • 低投入样本不再需要专家库建设工作流程或试剂
  • 不含PCR的工作流程可能只需50 ng原料
  • Both the existing and upgraded KAPA HyperPlus chemistry produce the highest post-ligation yields

Validated with theHyperCap工作流

KAPA-HyperPlus试剂盒测序结果优越

  • Higher post-ligation yields result in fewer amplification cycles and lower duplication rates
  • 使用升级版的KAPA-HyperPlus试剂盒,由于测序伪影的减少和灵敏度的提高,可以高置信度检测低频突变

Improved coverage depth for FFPE samples compared to Covaris-sheared DNA

  • Significant increase in coverage depth compared to KAPA HyperPrep Kits using Covaris-sheared DNA
  • 与使用Covaris剪切DNA的KAPA-HyperPrep试剂盒相比,复制率更低,靶向阅读更多,碱基覆盖更多
  • 使用Covaris剪切DNA和HyperPlus试剂盒检测从KAPA HyperPrep试剂盒制备的文库之间高度相关的变体*

最小序列覆盖偏差

  • 与其他酶切方法相比,序列偏差较小
  • 较少的偏差导致更均匀的测序覆盖率和降低测序成本

特色网络研讨会

NGS library preparation: Theory and practice
第3部分:使用KAPA HyperPlus工具包准备库的实例

Presenter: Dr. Maryke Appel, Roche

马上看

*文件中的数据。